DNA Synthesis Reagents and Columns
New England Biolabs, Inc. Deep Vent DNA Polymerase – 1000 units
Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3 5 proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100C.
New England Biolabs, Inc. Vent (exo-) DNA Polymerase – 1000 units
Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3 5 proofreading exonuclease activity associated with Vent DNA Polymerase. This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase.
BIOSEARCH TECHNOLOGIES INC INVERSE ABASIC CNA CPG LOW BUL
Inverse Abasic CNA CPG Low Bulk Density, 600 A, 80-90 µmol/g, BULK (g)
Cayman Chemical 5-methylthIo DMT 1mg
An analytical reference standard categorized as a tryptamine; intended for research and forensic applications
New England Biolabs, Inc. PURExpress (aa, tRNA) Kit – 10 reactions
PURExpress (aa, tRNA) Kit is a variation of the PURExpress In vitro Protein Synthesis Kit where the amino acids and tRNA are omitted from the translation mix.
Cayman Chemical Humn ActIvatr ProteIn1 Rep 96
Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryopreservation method yields high cell viability post-thaw and provides the convenience of immediately dispensing healthy reporter cells into assay plates. There is no need for intermediate preparatory steps such as the spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup.
New England Biolabs, Inc. Faustovirus Capping Enzyme - 2500u
Faustovirus Capping Enzyme (FCE) catalyzes the addition of N7-methylguanosine cap (m7G) to the 5 prime end of triphosphorylated and diphosphorylated transcripts producing Cap-0 RNA. FCE is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase guanylyltransferase and (guanine-N7)-methyltransferase. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing. The Cap-0 structure also promotes RNA stability and prevents inadvertent activation of innate immune responses triggered by triphosphorylated RNA. FCE retains significant capping activity at low temperatures and tolerates reaction temperatures up to 55C. In many cases 1 uL of FCE (25 units) can cap over 100 ug of RNA in 1 hour at 37C. GTP and S-adenosylmethionine (SAM) are required for capping activity and are included with the enzyme.
New England Biolabs, Inc. Faustovirus Capping Enzyme - 500u
Faustovirus Capping Enzyme (FCE) catalyzes the addition of N7-methylguanosine cap (m7G) to the 5 prime end of triphosphorylated and diphosphorylated transcripts producing Cap-0 RNA. FCE is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase guanylyltransferase and (guanine-N7)-methyltransferase. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing. The Cap-0 structure also promotes RNA stability and prevents inadvertent activation of innate immune responses triggered by triphosphorylated RNA. FCE retains significant capping activity at low temperatures and tolerates reaction temperatures up to 55C. In many cases 1 uL of FCE (25 units) can cap over 100 ug of RNA in 1 hour at 37C. GTP and S-adenosylmethionine (SAM) are required for capping activity and are included with the enzyme.